Here is a selection of antibodies we have tested using the Antibody Amplifier™.
Some have never worked, or worked poorly, using the traditional sedentary ('dropping on')
method. We will add new antibodies to this site as they are tested.
Should you have any questions regarding any of the ProHisto products,
please contact us at info@prohisto.com.
Click Here for our Protocols.
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Antibody |
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| Anti-Nitrotyrosine 1:10,000 (Stressmarq Biosciences, Inc., Mouse Monoclonal, clone 39B6, cat# SMC-154). Recommended dilution for sedentary method: 1:1000. Mouse Colon Section from mouse with colitis. Paraffin embedded. |
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100 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti P21WAF1 1:2000 (Dako, Monoclonal, SX108).
Recommended Dilution for sedentary method:
1:25. Mouse Thymus Section, paraffin embedded. |
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80 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-p23, 1:10,000 (Stressmarq Cat# SMC-156). Recommended Dilution for sedentary method: Not Determined. Human colon carcinoma, paraffin embedded. |
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| Anti-p38alpha MAP Kinase, 1:10,000 (Stressmarq
Biosciences, Inc. Monoclonal, Clone 9F12, Cat#
SMC-152). Recommended Dilution for sedentary
method: Not Determined. Human colon cancer, paraffin
embedded. |
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| Anti-p44/p42 MAP Kinase 1:1,000 (Cell Signaling Technologies, Polyclonal, Cat#9102). Recommended Dilution for sedentary method: 1:25. Human breast cancer section. |  |
40 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-p53 1:1,000,000 (YES, 1 IN 1 MILLION!!) (Exalpha, Biologicals, Inc. Monoclonal, mouse clone Pab122, cat# X1494M). Recommended dilution for sedentary method: 1:1000. Mouse Colon Section from mouse with colitis. Paraffin embedded. |
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1000 FOLD DILUTION FROM SEDENTARY |
| Anti-PAD2 (peptidylarginine deiminase-2) 1:10,000 (Novus,
Monoclonal, Clone 4D4, Cat# H00011240-M01). Company
Recommended Dilution for sedentary method: Not
Recommended for IHC. Mouse colitis section, paraffin embedded. |
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The Manufacturer does not recommend for IHC. |
| Anti-PAD4 (peptidylarginine deiminase-4) 1:20,000 (Novus, Monoclonal, Cat# H00023569-M01). Company Recommended Dilution for sedentary method: Not Recommended for IHC. Mouse colitis section, paraffin embedded. |  |
The Manufacturer does not recommend for IHC. |
| Anti-Pan-PAD (peptidylarginine deiminase) 1:20,000 (Novus, Polyclonal, Rabbit, Cat# NB100-1853). Recommended Dilution for sedentary method: 1:200. Mouse colitis section, paraffin embedded. |
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100 FOLD DILUTED FROM SEDENTARY METHOD |
| Anti-Phospho-Serine-15 53 1:20,000 (Mouse monoclonal, Cell Signaling, Clone 16G8,
cat#9286S). Recommended dilution for sedentary method: 1:100. A. Human Colon
Cancer; B. Mouse Colitis Section. Paraffin embedded 200 FOLD DILUTION FROM SEDENTARY METHOD |
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| Anti-PolB 1:1000 (Abcam, Polyclonal, for
sedentary method: 1:50. Mouse tumor
section, paraffin embedded. |
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20 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-PUMA 1:7500 (Cell Signaling, Polyclonal,
Cat#4976). Recommended Dilution for
sedentary method: 1:200. Mouse colon
section, paraffin embedded. |
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FROM SEDENTARY METHOD |