Here is a selection of antibodies we have tested using the Antibody Amplifier™.
Some have never worked, or worked poorly, using the traditional sedentary ('dropping on')
method. We will add new antibodies to this site as they are tested.
Should you have any questions regarding any of the ProHisto products,
please contact us at info@prohisto.com.
Click Here for our Protocols.
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Antibody |
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| Anti-hsc70 (hsp73), 1:100,000 (Stressmarq Biosciences, Inc., Rabbit Polyclonal, Cat# SPC-102). Recommended Dilution for sedentary method: Not Determined. Human colon cancer, paraffin embedded. |  |
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| Anti-HSF1, 1:10,000 (Stressmarq Cat# SPC-118). Recommended Dilution for sedentary method: Not Determined. Human colon carcinoma, paraffin embedded. |
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| Anti-Hsp25/27, 1:100,000 (Stressmarq Biosciences, Inc., Mouse Monoclonal, Clone 8A7, Cat# SMC-114). Recommended Dilution for sedentary method: Not Determined. Human colon cancer, paraffin embedded. |  |
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| Anti-Hsp60, 1:100,000 (Stressmarq Biosciences, Inc.,
Mouse Monoclonal, Clone LK-1, Cat# SMC-110).
Recommended Dilution for sedentary method: Not
Determined. Human colon cancer, paraffin embedded. |
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| Anti-Hsp60, 1:100,000 (Stressmarq Biosciences, Inc.,
Mouse Monoclonal, Clone LK-1, Cat# SMC-111).
Recommended Dilution for sedentary method: Not
Determined. Human colon cancer, paraffin embedded. |
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| Anti-HSP70, 1:10,000 (Stressmarq Cat# SMC-100).
Recommended Dilution for sedentary method:
Not Determined. Human colon cancer, paraffin embedded. |
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| Anti-Hsp70, 1:50,000 (Stressmarq Biosciences, Inc., Rabbit
Polyclonal, Cat# SPC-103). Recommended Dilution for
sedentary method: Not Determined. Human colon cancer,
paraffin embedded. |
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| Anti-HSP70/HSC70, 1:10,000 (Stressmarq Biosciences, Inc. Monoclonal, clone BB70, Cat# SMC-106). Recommended Dilution for sedentary method: Not Determined. Human colon cancer, paraffin embedded. |  |
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| Anti-HSP90, 1:20,000 (Stressmarq Biosciences,
Inc. Monoclonal, clone H9010, Cat# SMC-107).
Recommended Dilution for Human colon cancer, paraffin embedded. |
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| Anti-Hsp90, 1:10,000 (Stressmarq Biosciences, Inc.
Recommended Dilution for sedentary method: Not
Determined. Human colon cancer, paraffin embedded. |
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| Anti-Hsp90 alpha, 1:10,000 (Stressmarq Biosciences, Inc. monoclonal, Cat# SPC-147). Recommended Dilution for sedentary method: Not Determined. Human colon carcinoma, paraffin embedded. |  |
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| Anti-Hsp90, 1:100,000 (Stressmarq Biosciences, Inc.,
Mouse Monoclonal, Clone 4F3,E8, Cat# SMC-149).
Recommended Dilution for sedentary method: Not
Determined. Human colon cancer, paraffin embedded. |
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| Anti-IFN-gamma 1:100,000 (Lifespan Biosciences, polyclonal, Cat#LS-C7639). Recommended Dilution for sedentary method: 1:500. Mouse colitis section, paraffin embedded. |
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200 FOLD DILUTED FROM SEDENTARY METHOD |
| Anti-iNOS 1:5000 (Cayman, Polyclonal, Cat#160862).
Recommended Dilution for sedentary method: Not
stated for IHC. Colon section from mouse with colitis, paraffin embedded. |
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PRODUCT INSERT DOES NOT SAY IT CAN BE USED WITH PARAFIN EMBEDDED TISSUE SECTIONS |
| Anti-iNOS 1:10,000 (Res & Diagnostic Antibodies, Monoclonal Clone 5D5-H7, Cat# MC-5245). Recommended Dilution for sedentary method: 1:50. Colon section from mouse with mild colitis, paraffin embedded. |  |
200 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-KDEL, 1:10,000 (Stressmarq Biosciences, Inc. monoclonal Cat# SMC-129). Recommended Dilution for sedentary method: Not Determined. Human colon carcinoma, paraffin embedded. |
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| Anti-Ki67 1:1,000,000 (YES, 1 IN 1 MILLION!!!) (Dako,
Monoclonal, MIB-1) Recommended Dilution for sedentary method: 1:75. Mouse colon section, paraffin embedded. |
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13,333 FOLD DILUTION FROM SEDENTARY METHOD |