The Antibody Amplifier™ improves IHC in 4 ways:
1.) Increases Antibody Dilution
2.) Promotes Even Staining
3.) Enhances Reproducibility
4.) Freeze and Re-use Antibody Multiple Times
These pictures depict a comparison of the "standard" i.e. "dropping on" method versus the PROHisto method using the Antibody Amplifier.
INCREASES ANTIBODY DILUTION
The above images show the improved efficiency with the Antibody AmplifierTM using less concentrated antibody. The antibody here was anti-phospho-pRb Ser 780 (polyclonal, Cell Signaling). These are paraffin-embedded, serial sections from mouse colon.
ENHANCED EVEN STAINING
The above images show the improved efficiency with the Antibody AmplifierTM using less concentrated antibody. The antibody here was anti-phospho-pRb Ser 780 (polyclonal, Cell Signaling). These are paraffin-embedded, serial sections from mouse colon. Figure. Shows the improved quality of staining and even staining patterns using the Antibody AmplifierTM. On the left is the standard sedentary method, where there is uneven staining. The right side of the picture shows that using the Antibody AmplifierTM results in even and reproducible staining. We used the anti-phospho-pRb Ser 780 (polyclonal, Cell Signaling). These are paraffin-embedded, serial sections from mouse colon. For the STANDARD method (left), the antibody concentration was 1:1000. For the Antibody AmplifierTM method (right) (rocking the slides), the antibody concentration was 1:100,000.
INCREASED REPRODUCIBILITY
The above images show increased consistency and improved reproducibility of staining with the Antibody AmplifierTM. These are serial sections. On the left, arrows indicate where there are clear differences in staining patterns in serial sections. Alternatively, the right side shows the consistent staining pattern using the Antibody AmplifierTM. We used the anti-phospho-pRb Ser 780 (polyclonal, Cell Signaling). These are paraffin-embedded, serial sections from mouse colon. For the STANDARD method (left), the antibody concentration was 1:1000. For the Antibody AmplifierTM method (right) (rocking the slides), the antibody concentration was 1:100,000.
REUSE YOUR ANTIBODY
Using the Antibody AmplifierTM, you can re-use antibodies up to 4 times without loosing efficiency. We used the anti-phospho-pRb Ser 780 (polyclonal, Cell Signaling, 1:100,000). These are paraffin-embedded, serial sections from mouse colon.
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