CONTACT
Phone: 803-407-0506
Fax: 803-407-0260
Email: prohisto@yahoo.com
Phone: 803-407-0506
Fax: 803-407-0260
Email: prohisto@yahoo.com
Here is a continued selection of antibodies we have tested using the Antibody Amplifier™.
Some have never worked, or worked poorly, using the traditional sedentary ('dropping on')
method. We will add new antibodies to this site as they are tested.
Some have never worked, or worked poorly, using the traditional sedentary ('dropping on')
method. We will add new antibodies to this site as they are tested.
FOR A DEMO, SEE:
DEMONSTRATION
DEMONSTRATION
Anti-Ubiquitin, 1:100,000 (Stressmarq
Biosciences, Inc. Polyclonal, Cat# SPC-119).
Recommended Dilution for sedentary method:
Not Determined. Mouse colon colitis, paraffin
embedded.
Biosciences, Inc. Polyclonal, Cat# SPC-119).
Recommended Dilution for sedentary method:
Not Determined. Mouse colon colitis, paraffin
embedded.
Anti-p38alpha MAP Kinase, 1:10,000 (Stressmarq
Biosciences, Inc. Monoclonal, Clone 9F12, Cat#
SMC-152). Recommended Dilution for sedentary
method: Not Determined. Human colon cancer, paraffin
embedded.
Biosciences, Inc. Monoclonal, Clone 9F12, Cat#
SMC-152). Recommended Dilution for sedentary
method: Not Determined. Human colon cancer, paraffin
embedded.
Anti-CD74, 1:10,000 (Stressmarq Cat# SPC-116).
Recommended Dilution for sedentary method:
Not Determined. Human colon cancer, paraffin
embedded.
Recommended Dilution for sedentary method:
Not Determined. Human colon cancer, paraffin
embedded.
Anti-Calnexin-NT, 1:10,000 (Stressmarq Biosciences,
Inc. Polyclonal, Cat# SPC-127). Recommended
Dilution for sedentary method: Not Determined.
Mouse Colon, paraffin embedded.
Inc. Polyclonal, Cat# SPC-127). Recommended
Dilution for sedentary method: Not Determined.
Mouse Colon, paraffin embedded.
In addition to IHC, the Antibody Amplifier™ can be used for immunoflourescence. It's sturdy
construction and snap-on lid make the transportation of slides from hood to benchtop to microscope
easy and eliminates the worry of reagent run-off. Again, because the slides are immersed in dye
solution instead of "dropping on", your confidence in final results is greatly increased.
construction and snap-on lid make the transportation of slides from hood to benchtop to microscope
easy and eliminates the worry of reagent run-off. Again, because the slides are immersed in dye
solution instead of "dropping on", your confidence in final results is greatly increased.
IMMUNOFLOURESCENCE
A ) THIS IS THE DETECTION OF REACTIVE OXYGEN SPECIES IN STIMULATED LIVE MOUSE MACROPHAGES. THE GREEN
COLOR IS THE FLOURESCENT DYE 5-CARBOXY-2'-7'-DICHLORODIHYDROFLOURESCEIN DIACETATE.
COLOR IS THE FLOURESCENT DYE 5-CARBOXY-2'-7'-DICHLORODIHYDROFLOURESCEIN DIACETATE.
B) THESE ARE THE ABOVE LIVE MOUSE MACROPHAGES, STAINED WITH THE NUCLEIC ACID DYE, HOECHST
33342;
33342;
Anti-p53 Pab122, diluted at 1:1,000,000 using a
greatly diluted Alexa Fluor 568 labeled secondary
antibody. Mouse colon from mouse with colitis
greatly diluted Alexa Fluor 568 labeled secondary
antibody. Mouse colon from mouse with colitis
A)
B)
Anti-Hsp90 alpha, 1:10,000 (Stressmarq Biosciences,
Inc. monoclonal, Cat# SPC-147). Recommended
Dilution for sedentary method: Not Determined.
Human colon carcinoma, paraffin embedded.
Inc. monoclonal, Cat# SPC-147). Recommended
Dilution for sedentary method: Not Determined.
Human colon carcinoma, paraffin embedded.
Anti-FKBP51, 1:10,000 (Stressmarq Biosciences,
Inc., monoclonal Cat# SMC-139). Recommended
Dilution for sedentary method: Not determined.
Human breast carcinoma, paraffin embedded.
Inc., monoclonal Cat# SMC-139). Recommended
Dilution for sedentary method: Not determined.
Human breast carcinoma, paraffin embedded.
Anti-acetylated lysine, 1:100,000 (Stressmarq
Biosciences, Inc. monoclonal Cat# SMC-153).
Recommended Dilution for sedentary method: Not
Determined. Human GI tissue, paraffin embedded.
Biosciences, Inc. monoclonal Cat# SMC-153).
Recommended Dilution for sedentary method: Not
Determined. Human GI tissue, paraffin embedded.
Anti-p23, 1:10,000 (Stressmarq Cat# SMC-156).
Recommended Dilution for sedentary method:
Not Determined. Human colon carcinoma,
paraffin embedded.
Recommended Dilution for sedentary method:
Not Determined. Human colon carcinoma,
paraffin embedded.
Anti-AHA-1, 1:100,000 (Stressmarq Biosciences,
Inc. polyclonal). Recommended Dilution for
sedentary method: Not Determined. Mouse colitis
section, paraffin embedded.
Inc. polyclonal). Recommended Dilution for
sedentary method: Not Determined. Mouse colitis
section, paraffin embedded.
Anti-AHA-1, 1:100,000 (Stressmarq Biosciences,
Inc. polyclonal). Recommended Dilution for
sedentary method: Not Determined. Human GI
tissue, paraffin embedded.
Inc. polyclonal). Recommended Dilution for
sedentary method: Not Determined. Human GI
tissue, paraffin embedded.
Anti-ERK 1/2, 1 in 25,000 (ProHisto, LLC, Catalog #:
PA50). Mouse spleen, C.parvum-treated.
Immunofluorescence.
PA50). Mouse spleen, C.parvum-treated.
Immunofluorescence.
Anti-HSP70, 1 in 50,000 (ProHisto, LLC, Catalog #:
PA60). Mouse spleen, C.parvum-treated.
Immunofluorescence.
PA60). Mouse spleen, C.parvum-treated.
Immunofluorescence.
Anti-PUMA, 1:10,000 (Prohisto, LLC Catalog #:
PA100). Mouse spleen, C.parvum-treated.
Immunofluorescence.
PA100). Mouse spleen, C.parvum-treated.
Immunofluorescence.
Anti-Ubiquitin, 1:100,000 (Prohisto, LLC Catalog #:
PA110). Mouse spleen, C.parvum-treated.
Immunofluorescence.
PA110). Mouse spleen, C.parvum-treated.
Immunofluorescence.
Anti-Toll-Like-Receptor- 4, 1:20,000
(Stressmarq Biosciences, Inc. Polyclonal, Cat#
SPC-200). Mouse colitis, paraffin embedded.
(Stressmarq Biosciences, Inc. Polyclonal, Cat#
SPC-200). Mouse colitis, paraffin embedded.
Anti-Calnexin-CT, 1:100,000 (Stressmarq
Biosciences, Inc. Polyclonal, Cat# SPC-200).
Mouse colitis, paraffin embedded.
Biosciences, Inc. Polyclonal, Cat# SPC-200).
Mouse colitis, paraffin embedded.
